Sequence Annotation on Genome Compiler
Sequence annotation can be done very easily with the auto-annotation feature in Genome Compiler, which significantly reduces the time you need to dedicate to annotating your sequences. But for those who still prefer the precision of manual annotation, you can do that using the software as well.
Here is an example of the manual sequence annotation process on Genome Compiler of the vector pET28a:
- Begin with uploading the sequence you would like to annotate. You can do so by searching for a specific sequence on the Materials Box, upload a file from your computer, direct search from NCBI on Genome Compiler, etc. One you've uploaded the sequence, the software will ask you if you would like to auto-annotate it, so in case you'd like to go for the manual annotation, just press 'No'.
- To manually add an annotation, highlight the region you would like to annotate, right click, and select “annotate selection” from the menu.
- This will open the “more properties” tab on the right, where you can name your part, set a feature key, designate the strand, and add notes to your annotation.
- To create a sub annotation, highlight the sequence, right click, and select “add sub annotation.” Sub annotations appear below the main annotation in the circular view. The highlighted area of the sequence above shows how your sub annotation appears in the sequence view. You can annotate your sub annotations from the annotations summary table.
Check out this webinar for a more detailed explanation of both of the manual and the auto sequence annotation processes on Genome Compiler.